Daphne Rosen was born in Tel Aviv, Israel and later moved to Boston, Massachusetts with her family when she was three. She then began modeling when she was 18 years old. She found this hard at first, but then adapted to the change.
After modeling for SCORE magazine and appearing on The Jenny Jones Show, she entered the porn industry at the age of 20 and worked for many hardcore production companies in San Fernando Valley, California and in Miami, Florida. She first appeared on screen as the box covergirl of Hustler's Busty Beauties #7 and shortly after that, appeared on the cover of the November 2003 Big Butt magazine, followed by revealing appearances in other adult magazines. She also helped discover Score model Eve Tyler.
Daphne Rosen has performed in over 150 pornographic movies in over two years. She has signed a contract for Venom Digital Media, became an on-air personality for KSEX Radio, starred in Vivid Entertainment's Bare Naked, Beaver Pleasers and, along with Flower Tucci, a recurring role as The Party Girls in The End Game, The American Dream and various other films for Vivid Entertainment, appeared alongside comedian Rob Schneider on Playboy Radio, appeared on the television show Blind Date (as a date) and headlined in Elizabeth Starr's AVN award-winning remake of Russ Meyer's classic Faster, Pussycat! Kill! Kill! Kill! Kill! (titled Faster Pussycat! Fuck! Fuck! Fuck! Fuck!) opposite porn icon/actor Ron Jeremy and Tiffany Towers, which earned the AVN Award for best Big Busts on film. This was the third year in a row that the Big Bust AVN award-winning movie has starred Daphne Rosen. Rosen also presented the biggest award of the night, for best picture, alongside Lauren Phoenix.
She has co-hosted a weekly internet radio show, Two Live Jew with Daphne Rosen & Harry Weiss, on KSEX Radio. In late-2005, she had temporarily backed away from performing sex on camera to focus on her adult entertainment businesses. In June 2006, she returned to the adult world to film a self-themed feature with Score. On August 31, 2006, she and Weiss announced that they are leaving KSEX, on good terms, due to Rosen's increasingly busy schedule since returning to the world of porn and to start her feature dancing career.
Daphne Rosen is currently managed by former feature dancer Lisa Ann for films and represented by the Pure Talent Agency for feature dancing.